Start2Fold

The database of hydrogen/deuterium exchange data on protein folding and stability

Entry STF0052

Nanobody from Lama glama

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Protein information

Name of the protein: H14 protein
Organism: Lama glama (Llama)
Number of residues: 117
Related UniProt entry:   A2KD53 (Fragment: 2 - 117)
Related PDB entry:   1HCV

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Experiment sets

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STRONG

Method: Native exchange NMR

Conditions: pH 4.6; 27.0 Celsius; Probes: 54

Related publication:
 PMID 11141058

Experiment details: "For the hydrogen-deuterium exchange experiments, lyophilized, uniformly 15N-labeled VHH-H14 was dissolved in D2O buffer [10 mM deuterated sodium acetate (99.5% D), 100 mM NaCl, and 0.02% sodium azide (pH 4.6)] to a concentration of 0.5 mM. The pH meter readings were corrected for the isotope effect."

Protection threshold: log(P) > 4

Sequence: DVQLQESGGGLVQAGGSLRLSCAASGRTGSTYDMGWFRQAPGKERESVAAINWDSARTYYASSVRGRFTISRDNAKKTVYLQMNSLKPEDTAVYTCGAGEGGTWDSWGQGTQVTVSS
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STRONG residues

18: L; 20: L; 34: M; 35: G; 36: W; 37: F; 38: R; 49: A; 51: I; 69: T; 71: S; 80: Y; 81: L; 82: Q; 83: M; 87: K; 90: D; 91: T; 92: A; 94: Y; 97: G; 109: Q;
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MEDIUM

Method: Native exchange NMR

Conditions: pH 4.6; 27.0 Celsius; Probes: 54

Related publication:
 PMID 11141058

Experiment details: "For the hydrogen-deuterium exchange experiments, lyophilized, uniformly 15N-labeled VHH-H14 was dissolved in D2O buffer [10 mM deuterated sodium acetate (99.5% D), 100 mM NaCl, and 0.02% sodium azide (pH 4.6)] to a concentration of 0.5 mM. The pH meter readings were corrected for the isotope effect."

Protection threshold: 2 < log(P) < 4

Sequence: DVQLQESGGGLVQAGGSLRLSCAASGRTGSTYDMGWFRQAPGKERESVAAINWDSARTYYASSVRGRFTISRDNAKKTVYLQMNSLKPEDTAVYTCGAGEGGTWDSWGQGTQVTVSS
 CLICK TO DOWNLOAD SEQUENCE IN FASTA

MEDIUM residues

12: V; 14: A; 16: G; 17: S; 21: S; 23: A; 46: E; 48: V; 50: A; 52: N; 61: A; 68: F; 84: N; 93: V; 95: T; 99: G; 106: S; 108: G; 113: V; 114: T; 115: V;
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